The dynamics of Earth’s D” layer at the base of the mantle plays an essential role in Earth’s thermal and chemical evolution. Mantle convection in D” is thought to result in seismic anisotropy; therefore, observations of anisotropy may be used to infer lowermost mantle flow. However, the connections between mantle flow and seismic anisotropy in D” remain ambiguous. Here we calculate the present-day mantle flow field in D” using 3D global geodynamic models. We then compute strain, a measure of deformation, outside the two large-low velocity provinces (LLVPs) and compare the distribution of strain with previous observations of anisotropy. We find that, on a global scale, D” material is advected towards the LLVPs. Strain is highest at the core-mantle boundary (CMB) and decreases with height above the CMB. Material outside the LLVPs mostly undergoes lateral stretching, with the stretching direction often, but not always, aligning with mantle flow direction. Strain generally increases towards the LLVPs and reaches a maximum at their edges, although models that consider recrystallization suggest that anisotropy may actually be weaker near LLVP edges. The depth-averaged strain in D” is >1.5 in almost all regions, consistent with widespread observations of seismic anisotropy. The mantle flow field and strain in D” outside of LLVPs are not very sensitive to LLVP density but are strongly controlled by local density and viscosity variations outside the LLVPs. Flow directions inferred from anisotropy observations often (but not always) align with predictions from geodynamic modeling calculations.