Genomic DNA extraction
Genomic DNA (gDNA) was extracted from approximately 100 mg of each fecal
sample using the ZymoBIOMICSTM DNA Miniprep Kit (Zymo
Research, U.S.), according to the manufacturer’s protocol. A
NanoDropTM spectrophotometer was used to quantify and
assess the purification of the gDNA. The expected real-time quality
score had to be > 7. However, the results obtained
using the given protocol showed low DNA concentrations and inadequate
purification for amplification. Therefore, we adjusted the protocol to
achieve a better yield and more highly purified gDNA (See supplement).