CONCLUSION
We used RenSeq to sequence the R gene libraries of S.
integrifolium plants from across a rainfall gradient to evaluate the
hypothesis that CMI imposes a biotic stress whose evolutionary pressure
should be evident within the genome. We detected a significant positive
relationship between CMI and R gene count, supporting our hypothesis. We
demonstrate that RenSeq can be used in the evaluation of ecological
hypotheses and show promise for the economical interrogation of the
specific R gene loci responsible for disease resistance.