Fig.S1:
Differential gene expression and pathway analysis in MHV-1 vs
mock-infected lean and obese C3H/HeJ mice.
RNAseq was performed on the RNA collected from blood of MHV-1 and
mock-infected lean and obese C3H/HeJ mice at 2 dpi. (A & B)
Volcano plot of differentially expressed genes (DEGs) for lean (A) and
obese (B) mice. For identification of DEGs, genes with p-adjusted
values < 0.1 and log2FoldChange >
1, were selected by integrated Differential Expression and Pathway (iDEP
.96) analysis tool. (C & D) Pathway analysis for lean (C) and
obese (D) mice. Pathways were generated using Gene Set Enrichment
Analysis (GSEA) using the Kyoto Encyclopedia of Genes and Genomes
(KEGG). All the pathway analysis data were compared to data from the
blood of COVID-19 patients with severe disease; orange arrows indicate
common upregulated and blue arrows indicate common downregulated
pathways between MHV-1 infected vs mock and severe vs mild COVID-19
patients and the common up or downregulated pathways are represented by
arrows.