RNA extraction from whole blood
Mice were anesthetized with ketamine and xylazine (90 mg/kg and 5 mg/kg respectively) intraperitoneally and 100 µL of whole blood was collected via submandibular cheek bleeds, in EDTA coated tubes. We added 300 µL of TRIzol LS reagent (ThermoFisher) to each tube and total RNA was extracted using the manufacturer’s protocol and stored at -80°C until use.