Haemorrhage and Transfusion
After 30 minutes of anaesthetic equilibration and baseline arterial
blood pressure recordings from each site, blood was removed in 5 mL/kg
bwt increments up to 25 mL/kg bwt via continuous suction from jugular
catheters and stored in sterile bags with the appropriate volume of
citrate-phosphate-dextrose-adenine (14 ml CPDA-1 per 100 mL blood). Ten
minutes after each incremental blood removal, MAP readings obtained via
NIBP at the base of the tail, or invasively from the three
aforementioned peripheral sites (n = 9 horses) and the thoracic
aorta (n = 4 horses) were recorded. Blood was returned in the
same 5 mL/kg aliquots and repeated until the full 25 mL/kg was
administered. At the conclusion of the experiment, horses were not
recovered from anaesthesia and were euthanized by administration of
intrathecal lidocaine (VetOneĀ®, 4 mg/kg bwt).16