Expression of candidate miRNA from literature
Expression profiles of candidate miRNAs identified as differentially expressed between direct developing and diapausing pupae of the flySarcophaga bullata (Reynolds et al., 2017), pharate larvae of the mosquito Aedes albopictus (Batz et al., 2017), and pupae of the moth Helicoverpa zea (Reynolds, Nachman, & Denlinger, 2019) were checked for differential expression during diapause in both tissues ofP. napi . Combined, this comprised 16 candidate miRNA genes: miR-1-3p, miR-9c-5p, miR-13b-3p, miR-14-5p, miR-31a-5p, miR-92b-3p, miR-275-3p, miR-276a-3p, miR-277-3p, miR-282, miR-283-5p, miR-286b-3p, miR-289-5p, miR-305-5p, miR-2942-3p, and bantam-5p.