Detection of Glycosides
Approximately 2 mg of ethanolic extract was shaken with 10ml of water in
a test tube, filtered, and the filtrate concentrated. To the filtrate,
2-3 drops of Molisch’s reagent was added together with 2ml of
concentrated sulphuric acid, carefully through the side of the test
tube. Reddish violet ring appear, indicating the presence of glycosides.