Drug treatment and assessment of TNBS-induced colitis
For the induction of TNBS-induced experimental colitis, C57BL/6 mice were randomly divided into three groups, including a normal group, a vehicle control group, and a drug treatment group that received 2.5% 2,4,6-trinitro-Benzenesulfonic acid (TNBS) by clysma on the first day of the experiment. The drug treatment group received 40mg/kg PHI orally. The body-weight loss ratio and survival rate were monitored. At the end of the experiment, mice were anesthetized using intraperitoneal injection of 4 % chloral hydrate. The peripheral blood, colon, and mesenteric lymph nodes were collected for subsequent analysis.