2.1.3 The physicochemical and basic biological characteristics of the W541vaccine protein
The physicochemical properties of the vaccine proteins, including amino acid composition, molecular weight, theoretical isoelectric point (pI), instability index (II), aliphatic index (AI), and grand average of hydropathicity (GRAVY), were predicted using the ProtParam server(39). The SignaIP 4.1 server (40), MHMM 2.0 server, and Cell-PLoc 2.0 server(41) were used to predict the presence of signal peptide sequences, transmembrane regions, and subcellular localization of the vaccine proteins after expression within host cells, respectively. The antigenicity, allergenicity, and toxicity of the vaccine proteins were predicted using the VaxiJen server(42), AllerTop server(39), and ToxinPred server(43), respectively. Human homology analysis on the vaccine proteins was performed using the BLAST server(44).