Conclusion
MTB infection caused significant up-regulation of catabolism-related DE genes, GO biological processes, and signal pathways in mice, and significant down-regulation of anabolism-related DE genes, GO biological processes, and signal pathways, as well as significant down-regulation of multiple immune-related pathways. Our study found for the first time that the effective doses of ag85a/b DNA vaccine immunized whether by IM or EP could significantly up-regulate immune-related pathways, and recover the metabolic disorder and the injury caused by MTB. The action target and mechanism of two effective treatment groups (100μg DNA IM group and 50μg DNA EP group) are highly consistent. These findings provide a basis for further elucidating the immunotherapeutic target and mechanism of the ag85a/b DNA vaccine.