Differential gene screening and identification
DE genes after treatment with different doses of ag85a/b DNA vaccine immunized with IM or EP were screened by a hierarchical clustering heat map, scatter plot, and volcano plot analysis. The DE genes were compared between the TB model group and the normal control group and between each ag85a/b DNA treatment group and the TB model group. A Hierarchical cluster plot shows differences in gene expression among different groups of samples. The scatter plot shows the DE gene correlation between the two groups of samples, where the x-axis values and the y-axis values are mean-normalized (log2 scaling) processed results. The volcano plot shows the distribution of DE genes between the two groups of samples, and the x-axis represents the log2 value (Fold Change), and the y-axis represents the -log10 value(P-value).