GO analyses of Differentially expressed genes.
The significant DE genes between the TB model group and the normal group
or each ag85a/b DNA vaccine group and TB model group were
subjected to GO terms for the BP, CC, and MF by GO analyses, and the top
10 significant BP, CC, and MF items enriched in each group were compared
(Supplementary Fig. 2). The results showed that: (1) In the TB model
group, the up-regulated BP items were mainly enriched in the secretion
and regulation of cells, proteins, insulin and hormones, etc; the
down-regulated BP items were mainly enriched in locomotion,
developmental process, and cell migration, etc. The up-regulated CC
items were mainly enriched in the host cell cytoplasm, host
intracellular part, neuronal cell body, and secretory granule, etc; the
down-regulated CC items were mainly enriched in extracellular matrix
part, extracellular region, plasma membrane, and the vesicle, etc. The
up-regulated MF items were mainly enriched in ligand activation,
G-protein coupled receptor binding, calcium ion binding, peptidase
activity, and hormone activity, etc. the down-regulated MF items were
mainly enriched in binding, protein binding, ion binding, and receptor
binding, etc. (2) Among the top 10 significantly up-regulated or
down-regulated GO items in the TB model group, the 10μg and 50μg DNA IM
groups had little effect on them, most up-regulated GO items were
significantly down-regulated, and most down-regulated GO items were
significantly up-regulated in 100μg, 200μg DNA IM groups, and 4 DNA EP
groups.