Cell culture
Mice melanoma cell line B16F10 were grown in Roswell Park Memorial
Institute 1640 (RPMI 1640) medium (Lonza, Verviers, Belgium)
supplemented with 10% FBS (Lonza, Verviers, Belgium), 10 units of
penicillin, 10 mg of streptomycin, 25 µg/mL of amphotericin B
(Sigma-Aldrich, Saint-Louis, USA), and 1% non-essential amino acids
(Lonza, Verviers, Belgium). Cell lines were cultured and maintained at
37 °C in a humidified atmosphere with 5% CO2.