Plasma and Brain pharmacokinetics studies of crocetin
The plasma and brain pharmacokinetics studies were carried out at 10 mg kg-1 (p.o.). Crocetin was given in the form of a liquid formulation similar to the one used during the treatment of animals for other studies. The formulation contained 10% gelatine, 1% sucralose and 25 mg ml-1 of crocetin. The formulation was kept at 37 °C to maintain its fluidity and administered orally via cannula at a concentration of 10 mg kg-1 of crocetin. Bood and Brain samples were taken at six different time points for up to 8 h of study. The quantitative analysis of crocetin present in blood plasma and brain was done by using LC-MS/MS method. The analysis of pharmacokinetic parameters was done by using PK solution software, Summit Research Services, USA.