Neuromuscular coordination tests
The neuromuscular coordination of mice was recorded by using two different methods; The rotarod, and wire hanging test. For both the tests, mice were trained for three consecutive days with three trials per day. The maximum time allowed on the rotarod or wire mesh during the experiment was 300 and 120 seconds respectively. The rotarod was initially set at a speed of 4 rpm at the start of the test and accelerated to 40 rpm over 300 seconds. The latency to fall from the rotarod or wire mesh was recorded for each mouse, and the mean latency to fall for each group was calculated.