Figure 6. Crocetin upregulated the energy levels of other vital organs of the body and enhanced the median life span of male mice.
Quantification of energy metabolites NAD+, NADH, ATP, and AMP in vital organs i.e., (A) Lung, (B) liver, (C) Heart, and (D) Kidney by using LC-MS/MS method. (E) OGTT, after administration of glucose 1g kg-1 orally, the level of glucose was measured in the blood at different time points (F) Neuromuscular coordination was studied by examining latency to fall from an accelerating rod in the rotarod test (G) or elevated wire mesh in the wire hanging test (n=14) Data are shown as mean ± SEM and statistical significance was calculated by comparing the control and crocetin groups. p-values were calculated using a two-tailed unpaired student-t test and are shown separately for each bar graph. (H) Survival curves comparing control to crocetin-treated male mice (n=23). The p-values reflect outcome of log-rank (Mantel-Cox) test. The arrows at 480 days indicate the age at which crocetin treatment was initiated.