Figure 4. Crocetin treatment changed the hippocampi gene profile
of old mice to bring it closer to young untreated mice
(A) Hierarchical clustering of gene expression tree of the normalized
count of young (n=5), old (n=7), and crocetin (n=7) group (B) Normalized
count of mitochondrial genome-specific genes ND1, ND2, ND3, ND4, ND5,
and ND6 and Normalized count of nuclear genome-specific genes Bdnf,
Uqcrq, Gfpt1, Eno3, Gabbar2, and Drosha. (D) Comparison of expression of
ND5 and ND6 among young untreated, old untreated and old mice treated
with crocetin through western blotting. The adjusted p-value <
0.05 was used to identify the genes, which were differentially
upregulated in old treated mice and formed clusters closer to young
mice. Data presented in Figure 4C are mean ± SEM, and statistical
comparisons were made among the groups of young untreated, old
untreated, and old mice treated with crocetin. p-values were calculated
using a two-tailed unpaired student-t test and are shown for each bar
graph.