Figure 5. Aged primary astrocytes showed improved mitochondrial function after treatment with crocetin
(A) Flow-cytometry analysis of mitochondrial membrane potential with mitoTraker Red CMXRos (B) Confocal analysis of mitochondrial membrane potential with mitoTraker Red CMXRos, Mean Fluorescence Intensity (MFI) was calculated for individual mitochondrion by using CellPathfinder software (C) Western blot analysis for ND5 and ND6 after 15 days treatment of aged primary astrocytes. The blot quantification was done using ImageJ software, and data were normalized with ACTB. (D) Quantification of energy metabolites NAD+, NADH, ATP, and AMP in aged astrocytes by using LCMS. Data are Mean ± SEM, and p-values were calculated by using a two-tailed unpaired student-t test and are shown for each bar graph.