Animal Care and Handling
This study used 16–18 months old C57BL/6J mice. The mice were housed in
a temperature (22 ± 1°C) and humidity (40-60%) controlled room with a
12-h light/dark cycle and provided food and water ad libitum. Every 15
days, a proper record of their body weight and physical wellness was
maintained during the study. Animals were randomized on the basis of
their body weight. At the end of experiments, wherever required, animals
were sacrificed by using CO2 Inhalation method. All
experiments were conducted after the approval of the institutional
animal ethics committee.