Differential gene expression analysis
DESeq2 (v 1.38.1) package was used to analyze the differential gene
expression. The total feature count of less than 5 in a gene’s row was
removed. Differential expression analysis was performed on the remaining
22096 genes. Regularized Log Transformation (RLT) was used to normalize
the data. The unbiased approach was used for Principal Component
Analysis (PCA) and to visualize the distribution of clusters after the
crocetin treatment.