Figure 5. Aged primary astrocytes showed improved mitochondrial
function after treatment with crocetin
(A) Flow-cytometry analysis of mitochondrial membrane potential with
mitoTraker Red CMXRos (B) Confocal analysis of mitochondrial membrane
potential with mitoTraker Red CMXRos, Mean Fluorescence Intensity (MFI)
was calculated for individual mitochondrion by using CellPathfinder
software (C) Western blot analysis for ND5 and ND6 after 15 days
treatment of aged primary astrocytes. The blot quantification was done
using ImageJ software, and data were normalized with ACTB. (D)
Quantification of energy metabolites NAD+, NADH, ATP,
and AMP in aged astrocytes by using LCMS. Data are Mean ± SEM, and
p-values were calculated by using a two-tailed unpaired student-t test
and are shown for each bar graph.