Differential gene expression analysis
DESeq2 (v 1.38.1) package was used to analyze the differential gene expression. The total feature count of less than 5 in a gene’s row was removed. Differential expression analysis was performed on the remaining 22096 genes. Regularized Log Transformation (RLT) was used to normalize the data. The unbiased approach was used for Principal Component Analysis (PCA) and to visualize the distribution of clusters after the crocetin treatment.