Open-field test
The mice were acclimatized to the testing room for 30 minutes before the
experiment. The open field arena box (60 cm x 45 cm x 25 cm) made up of
white colored plastic was used for all the open field experiments.
Individual mice were placed in the centre of the open field arena and
were allowed to freely explore it for 5 minutes. The behavior of mice
was recorded using a video camera connected to AnyMaze software. The
following behavioral parameters were recorded: Total distance travelled
(cm), average speed (m s-1), time spent in the centre
area (sec), time spent in the corners (sec) and the number of rearing
events (counted manually after analyzing the recorded videos).