Statistical analysis
All the data of crocetin and control group except transcriptomic data
were analyzed using a two-tailed unpaired t-test, and a p-value of less
than 0.1 is considered statistically significant using the GraphPad
Prism software (version 9.0.0 (121)), with statistical significance
determined as Pā<ā0.05 and checked for normal distribution.
Outliers were excluded based on a Grubbs test with É value set at a 0.05
significance level. Transcriptomics data were analysed using R (4.2.0),
with Log 2-fold change (LFC) >0.3 in the gene expression
significant at adjusted p-value <0.05. The categories
significantly enriched (p-value < 0.05) were considered for GO
and KEGG analysis.