Confocal Microscopy
The aged primary astrocytes were seeded in the optical bottom 96 well
plate (Thermo-165305). Cells were treated with crocetin (25 µM) for 15
days, the media were changed every 4th day until the
15th day of treatment. Fresh media containing
mitoTraker Red CMXRos (Invitrogen-M7512) and bis-benzamide was added to
the cells after 15 days of treatment. After 30 min of incubation, images
were captured using CQ1(Yokogawa) confocal quantitative Image Cytometer.
Fifteen fields were captured from each treatment group at 40X
magnification. More than 1500 cells from each group were taken for
further analysis. The images were analyzed using cell pathfinder
software.