Figure 2. Analysis of differential gene expression in hippocampi of old mice after treatment with crocetin
Clustering of gene expression from hippocampi of mice. (A) Representation of gene expression in Principal Component (PC) space. Values in parenthesis indicate the percentage of variance explained by control (blue) and crocetin groups (red). (B) Volcano plot of genes from hippocampi of mice. The red dots represent significantly upregulated genes (115), and the blue dots show significantly downregulated genes (39). (C) Heat map of some of the representative genes, which were differentially upregulated in hippocampi of mice treated with crocetin in comparison to the control group (standardized expression level; red boxes represent high expression; blue boxes signify low expression (the complete list of differentially upregulated genes is given in the Supplementary Figure S3B). (D) Heat map of all the downregulated genes. (F) Heat map of all the upregulated genes of the mitochondrial genome in mice hippocampi. padj < 0.05 and |log2(fold change) | > log2(0.3) were considered to be statistically significant, n = 7 mice in each group.