Figure 6. Crocetin upregulated the energy levels of other vital
organs of the body and enhanced the median life span of male mice.
Quantification of energy metabolites NAD+, NADH, ATP,
and AMP in vital organs i.e., (A) Lung, (B) liver, (C) Heart, and (D)
Kidney by using LC-MS/MS method. (E) OGTT, after administration of
glucose 1g kg-1 orally, the level of glucose was
measured in the blood at different time points (F) Neuromuscular
coordination was studied by examining latency to fall from an
accelerating rod in the rotarod test (G) or elevated wire mesh in the
wire hanging test (n=14) Data are shown as mean ± SEM and statistical
significance was calculated by comparing the control and crocetin
groups. p-values were calculated using a two-tailed unpaired student-t
test and are shown separately for each bar graph. (H) Survival curves
comparing control to crocetin-treated male mice (n=23). The p-values
reflect outcome of log-rank (Mantel-Cox) test. The arrows at 480 days
indicate the age at which crocetin treatment was initiated.