Plasma and Brain pharmacokinetics studies of crocetin
The plasma and brain pharmacokinetics studies were carried out at 10 mg
kg-1 (p.o.). Crocetin was given in the form of a
liquid formulation similar to the one used during the treatment of
animals for other studies. The formulation contained 10% gelatine, 1%
sucralose and 25 mg ml-1 of crocetin. The formulation
was kept at 37 °C to maintain its fluidity and administered orally via
cannula at a concentration of 10 mg kg-1 of crocetin.
Bood and Brain samples were taken at six different time points for up to
8 h of study. The quantitative analysis of crocetin present in blood
plasma and brain was done by using LC-MS/MS method. The analysis of
pharmacokinetic parameters was done by using PK solution software,
Summit Research Services, USA.