Figure 2. Analysis of differential gene expression in hippocampi
of old mice after treatment with crocetin
Clustering of gene expression from hippocampi of mice. (A)
Representation of gene expression in Principal Component (PC) space.
Values in parenthesis indicate the percentage of variance explained by
control (blue) and crocetin groups (red). (B) Volcano plot of genes from
hippocampi of mice. The red dots represent significantly upregulated
genes (115), and the blue dots show significantly downregulated genes
(39). (C) Heat map of some of the representative genes, which were
differentially upregulated in hippocampi of mice treated with crocetin
in comparison to the control group (standardized expression level; red
boxes represent high expression; blue boxes signify low expression (the
complete list of differentially upregulated genes is given in the
Supplementary Figure S3B). (D) Heat map of all the downregulated genes.
(F) Heat map of all the upregulated genes of the mitochondrial genome in
mice hippocampi. padj <
0.05 and |log2(fold change) | > log2(0.3)
were considered to be statistically significant, n = 7 mice in each
group.