Neuromuscular coordination tests
The neuromuscular coordination of mice was recorded by using two
different methods; The rotarod, and wire hanging test. For both the
tests, mice were trained for three consecutive days with three trials
per day. The maximum time allowed on the rotarod or wire mesh during the
experiment was 300 and 120 seconds respectively. The rotarod was
initially set at a speed of 4 rpm at the start of the test and
accelerated to 40 rpm over 300 seconds. The latency to fall from the
rotarod or wire mesh was recorded for each mouse, and the mean latency
to fall for each group was calculated.