Working and spatial memory test in a radial-arm maze task
The eight-armed radial arm maze from UGO Basile was used to test working and spatial memory. The mice were acclimatized to the maze for three days before the experiment. For testing the spatial and the working memory, separate set of mice were used. Each mouse was trained to navigate the maze for three consecutive days. The mouse was placed on the end of one arm (entry arm). For working memory, a food reward (100 mg of chocolate cookies) was placed at the end of four randomly selected arms. The movement of mice was tracked with the help of automated AnyMaze software connected with a tracking camera. A choice was considered correct if the mice entered an arm containing the food reward. Similarly, for spatial memory tasks, one arm out of eight was baited with a food reward, and various parameters were recorded.