Serum cholesterol levels
Three weeks after stocking, five fish were randomly removed from each holding tank and blood was collected from their caudal arch using heparinized 1 mL syringes and 25-gauge needles. Plasma cholesterol levels were determined using a hand-held Accutrend Plus Meter and Accutrend Cholesterol test strips (Roche diagnostics, Risch-Rotkreuz, Switzerland).