Fibroblast Cell Line |
Primary Fibroblasts (Human/Mouse) |
Minimal
differentiation ability, but vigorous proliferation capacity in vitro. |
Difficult to obtain and cultivate, cannot be continuously passaged;
may lose functionality and stability during prolonged culture. |
Fibroblast activation, excessive ECM deposition, etc. |
|
Human Embryonic Lung Fibroblast Cell Line (MRC-5 Cell Line) |
Fibroblast-like morphology; maintains a normal diploid karyotype during
long-term in vitro expansion; genetically stable; mesenchymal
characteristics; exhibits highly dynamic cell protrusions and enhanced
migration and invasion potential. |
Senescence after a certain number of
passages,difficult to produce on a large scale compared to passage
cells. |
EMT, activation of signaling pathways, fibroblast
activation, etc. |
|
Lung Fibroblast Cell Line (HFL1 Cell Line) |
Normal karyotype, easy
to cultivate, onsiderable yield. |
Limited cell line with a finite
lifespan. |
|
|
Mouse Embryonic Fibroblast Cell Line (NIH3T3 Cell Line) |
Continuous
passaging, easy cultivation, easily obtainable. |
High contact
inhibition, subcloning. |
|