Cell Representative Species Advantages Limitations Pathogenic Mechanisms
Fibroblast Cell Line Primary Fibroblasts (Human/Mouse) Minimal differentiation ability, but vigorous proliferation capacity in vitro. Difficult to obtain and cultivate, cannot be continuously passaged; may lose functionality and stability during prolonged culture. Fibroblast activation, excessive ECM deposition, etc.
Human Embryonic Lung Fibroblast Cell Line (MRC-5 Cell Line) Fibroblast-like morphology; maintains a normal diploid karyotype during long-term in vitro expansion; genetically stable; mesenchymal characteristics; exhibits highly dynamic cell protrusions and enhanced migration and invasion potential. Senescence after a certain number of passages,difficult to produce on a large scale compared to passage cells. EMT, activation of signaling pathways, fibroblast activation, etc.
Lung Fibroblast Cell Line (HFL1 Cell Line) Normal karyotype, easy to cultivate, onsiderable yield. Limited cell line with a finite lifespan.
Mouse Embryonic Fibroblast Cell Line (NIH3T3 Cell Line) Continuous passaging, easy cultivation, easily obtainable. High contact inhibition, subcloning.