2.5 Extraction and evaluation of the solubility of the colorants
After 14 days of fermentation, each culture broth was separated from the
mycelial mass by filtering through ordinary filter paper. The mycelial
mass was washed with hexane (20 mL), ethyl acetate (20 mL) and methanol
(20 mL). Solvents were added sequentially to the cultures and vigorously
shaken in an orbital shaker (100 rpm/60 min). Extracts were filtered
through filter paper for the removal of cell debris. The culture
filtrates were subjected to successive extractions. The culture filtrate
and solvent (hexane and ethyl acetate) were placed in a separating
funnel (1:1, v/v) and mixed well. After separation, the solvent fraction
was collected.