FIGURES LEGENDS
Fig. 1. Phases of the extracts of different polarities (hexane, ethyl acetate, methanol) using the mycelial mass and broth of the eight isolates that produce the most intense colorants (Penicillium sp. P3SO332, Clonostachys sp. P2SO329, Penicillium sp.P3SO224, Penicillium sp. P3SO322, Phoma sp. P2SO127,Penicillium sp. P3SO133, Penicillium sp. P2SO334,Monascus sp. C02172R).
Fig. 2. The evolutionary history was inferred by using the maximum-Likelihood likelihood method and the Tamura-Nei (1993) model. The percentage of trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 12 nucleotide sequences. The green dots indicate the isolates from this study and the others are ITS sequences of colorant-producing fungi. There was a total of 738 positions in the final dataset. Evolutionary analyses were conducted in MEGA X v10.2.4.
Fig. 3. Effect of different carbon and nitrogen sources in the production of colorants with the three selected fungi.Penicillium gravinicasei P3SO332 (A, B);Clonostachys rosea P2SO329 (C, D); Penicilliumsclerotiorum P3SO224 (E, F). Different letters represent significant differences according to the Student’s t-test (p< 0. 05).
Fig. 4. Structure of sclerotiorin present in the EtOAc extract of the fungi P . sclerotiorum P3SO224 (1) and penicillic acid (2) present in the EtOAc extract of Clonostachys roseaP2SO329. *Structures designed using Chemdraw 19.0
Fig. 5. Polyester, silk and cotton fabrics dyed with crude colorants extracted from Clonostachys rosea P2SO329 (CR9),Penicillium gravinicasei P3SO332 (PG2) and Penicillium sclerotiorum P3SO224 (PS4).
Fig. 6. Color and aspect of cream formulations comprising the colorant extracts (0.05 mg per gram of the formulation) produced byPenicillium sclerotiorum P3SO224 (PS4 10 mg), Clonostachys rosea P2SO329 (CR9 14 mg), Penicillium gravinicasei P3SO332 (PG2 14 mg) and commercial colorant (yolk-yellow liquid food coloring - MIX).
Fig. 7. Soap formulation using colorant 1 (0.05 mg per gram) obtained from Penicillium sclerotiorum P3SO224. Commercial colorant (yolk-yellow liquid food coloring - MIX).