Fig.14
Masson and Picrosirius red staining
for determination of collagen synthesis in rat model on the
7th and the 14th day. (A) Typical
photographs of MTS and Picrosirius red staining at Day 7 and 14 of all
the groups. (Scale bar:200 μm). (B) The quantitative results of MTS on
collagen deposition. (C) The quantitative results of Picrosirius red
staining on the ratio of COL I/III. Data are expressed as the mean\(\pm\)SEM; n ¼ 3; *P < 0.05; **P < 0.01; ***P
< 0.001; ****P < 0.0001.
Conclusion
- In conclusion, given that a wound occurs at the same position, varying
incident angles can significantly influence collagen remodeling
behavior around the wound under uniform laser energy conditions. A
low-energy 1064nm fiber laser, operating at an energy density of
67.543J/mm2, can effectively expedite wound healing in rats. Among
various angles tested, a 60° incident angle proved to be the most
effective, achieving the fastest healing rate while ensuring optimal
collagen network densification and minimized scarring.
- In vivo observations revealed that low-energy continuous lasers with
an incident angle around 60° exhibited superior performance in
accelerating wound healing and limiting thermal damage. Furthermore,
histological and immunohistochemical analyses corroborated that a 60°
laser with an energy density of 67.543 J/cm2 could
enhance wound cell proliferation, mitigate inflammation, stimulate
angiogenesis, and facilitate optimal re-epithelialization.
- Consequently, low-energy continuous lasers with incident angles around
60° show considerable promise for promoting wound healing in clinical
settings. However, it is important to note that the clinical healing
of chronic wounds can often be significantly influenced by a patient’s
underlying health conditions. As such, our laboratory will continue
conducting comprehensive research into the effects of low-energy
lasers on wound healing in diabetic rats, with the ultimate goal of
transitioning this technology into clinical trials in the near future.
Statements & Declarations