1.3 General optical microscopy and staining observation
Suspended drops of dormant, non-dormant and dead chlamydospore were
prepared and added to the slide. The slide was covered and photographed
under an optical microscope. The purified spore suspension was placed in
a 2 mL centrifuge tube at 1500 r/min for 5 min, 2/3 of the supernatant
was removed, and the milk phenol cotton blue dye solution (Guangzhou
Exon Biotechnology Co., LTD.) was added for 5 min at room temperature,
and the spore suspension was absorbed, observed and photographed on a
slide. 0.9 mL of the spore suspension was added to 0.1 mL of 0.4%
Trypan blue dye solution, mixed evenly for 3 min, and observed under a
microscope. Take a 5 mL centrifuge tube and firstly add 1 mL MTT dye
solution, 1 mL PBS buffer solution, at the same time add 2.5 μL cobalt
chloride solution with a concentration of 2.5 mM, take 100 μL spore
suspension to be dyed and purified, swirl and mix for 1 min, and then
incubate at 27 ℃ for 24-36 h. The spore fluid was absorbed and observed
under an optical microscope.