1.4 Metabolic activity of dormant and non-dormant chlamydospore
To determine the level of metabolic activity of dormant and non-dormant
chlamydospore, FUN-1 fluorescent probe (Shanghai Yubo Biotechnology Co.,
LTD.) was used for staining. The chlamydospore suspension 20 μL was
placed on a clean slide, and the prepared 10 μmol/L FUN-1 (containing
2% glucose) was added to the slide and stained for 30 min at room
temperature, away from light. Firstly, the fluorescence intensity was
examined under a fluorescence microscope (PWS-Scan-F, Shanghai Powerscin
Biotechnology Co., LTD.), then the fluorescence intensity was excited
under a confocal laser scanning microscope (Leica, Beijing Derica
Biotechnology Co., LTD.) with a wavelength of 488 nm laser, and then
with a wavelength of 488 nm and 633 nm laser at the same time. The
fluorescence changes of the two types of spores were observed and
photographed after the images at the two wavelengths were superimposed.