1.6 Electron microscope
According to the published method [29], the above dormant and non-dormant chlamydospores were coated on cellulose film respectively, and incubated at 28 ℃ for 12 h, then fixed overnight with 2.5% glutaraldehyde solution (special for electron microscopy, Beijing Mycorrhizal Biological Company), and then treated with PBS (0.01 M, pH7.4) briefly rinsed, alcohol gradient dehydration, tert-butanol vacuum drying. Samples on the charged rubber were gold-plated fixed, and then were observed and photographed by using scanning electron microscope (SEM) (JEOLS-3400IV, Peabody, Massachusetts) under 15 KV voltage.
These dormant and non-dormant chlamydospores were fixed in 2.5% glutaraldehyde, then washed three times with PBS buffer (pH7.4), placed in 1% osmic acid for 2 h, and washed three times with PBS buffer. After gradient dehydration with 30%~90% acetone at room temperature, the ultrathin sections were prepared by penetration embedding and polymerization at 60 ℃ for 36 h according to the conventional process of electron microscopy. The sections were treated with lead citrate staining solution for 10 min, and then stained with uranium acetate for 30 min. After washing and drying, JEM-1230 transmission electron microscope (TEM) was used to observe and photograph.