Comparing telomere length assayed using WGS and qPCR
To quantify the relationship between telomere length calculated using
qPCR and WGS data we used a Pearson correlation. First, relative
telomere length measured by qPCR was correlated with mean telomere
length estimates calculated using Computel, K-seek, and TRIP. Then, to
evaluate the role of genome coverage to estimates of telomere length we
correlated telomere length measured by qPCR with re-assessed telomere
length accounting for genome coverage (See Equation 2) from K-seek and
TRIP.