Figure 3. Hydroxamate-family of HDACs inhibitors are potent inducers of transgenic expression.
A. Bar graph showing the mean % of mVenus+ cells acquired by flow cytometry at day 6 of culture in clones 16, 17, 18, 20, 21 and WT treated with DMSO or inhibitors in 96 wells liquid culture. B. mVenus mean fluorescence intensity curve plot over 5 days following inhibitors treatment in clones 16, 17, 18, 20, 21 and WT as measured using a microplate reader. mVenus fluorescence was acquired in area scan mode and normalized to the OD750 acquired in the same reading mode. Finally, the levels of mVenus fluorescence intensity in the WT control (calculated similarly) was subtracted of each sample. Error bars represent standard deviation of biological triplicates.