Figure 4. SAHA steadily increases transgene expression over culture period.
Flask liquid cultures of clones 16, 17, 18, 20, 21 and WT were treated with 5 µM of SAHA and grown for 12 days. The mean fluorescence intensity (MFI) of mVenus was assessed every day. A. The MFI was measured on the overall culture using a microplate reader. B and C. represent the MFI and the % of mVenus+ cells, respectively, measured across growth period using a flow cytometer