Figure 3. Hydroxamate-family of HDACs inhibitors are potent
inducers of transgenic expression.
A. Bar graph showing the mean % of mVenus+ cells
acquired by flow cytometry at day 6 of culture in clones 16, 17, 18, 20,
21 and WT treated with DMSO or inhibitors in 96 wells liquid culture. B.
mVenus mean fluorescence intensity curve plot over 5 days following
inhibitors treatment in clones 16, 17, 18, 20, 21 and WT as measured
using a microplate reader. mVenus fluorescence was acquired in area scan
mode and normalized to the OD750 acquired in the same
reading mode. Finally, the levels of mVenus fluorescence intensity in
the WT control (calculated similarly) was subtracted of each sample.
Error bars represent standard deviation of biological triplicates.