2.7. Mutation effect analysis
Mutation effect analysis was performed using DynaMut.43 To compute the stability of SARS-CoV-2 wild-type (WT) and mutated (mutant) proteins, the reference structures were retrieved from the RCSB database (https://www.rcsb.org/), with the following IDs: 7CMD (Nsp3); 6M2Q (Nsp5); 6M71 (RdRp); 6VXX (Spike) and were then uploaded to the DynaMut server (https://biosig.lab.uq.edu.au/dynamut/). At specific site, a point mutation was inserted. The effect in the form of total energies and vibrational entropy energies between wild-type and mutated proteins was recorded.