Feed Materials
The amikacin solutions were prepared by dissolving amikacin sulfate in water-for-injections (WFI) or deionized water at 45 mg/ml amikacin base and pH adjusted to 6.7 using NaOH.
The lipid solution were prepared by dissolving DPPC and cholesterol at a 2:1 weight ratio in 100% ethanol at 20mg/ml.
The buffer solution consists of 1.5% NaCl in water-for-injections (WFI) or deionized water.