Phylogenetic reconstructions
Nucleotide sequences were translated and aligned using the MUSCLE
algorithm implemented in MEGA. Sequences (147 to 396 nucleotides)
generated in this work have been deposited in GenBank with the following
accession numbers:OR493270-OR493385.
For type characterization, phylogenetic trees were constructed using
ModelFinder, tree reconstruction and ultrafast bootstrap (1000
replicates) with IQ-TREE 2.1.3. RSV reference sequences used were in
File S2. The best-fit nucleotide substitution model GTR+F+G4 was
identified according to Bayesian information criterion. Bootstrap values
were estimated using the SH test and ultrafast bootstrap with 1000
replicates. To identify the phylogenetic relationship, 8373 G gene
sequences type A and 5339 type B available in the GenBank database (one
sequence of each type used for the Basic Local Alignment Search Tool
(BLAST) in March 2023) were used (File S2). Transmission clusters were
defined as viral sequences circulating in Asturias grouped into a single
and well supported monophyletic clade with >90% bootstrap
values. To search for mutations, the sequences were compared with ON1
(JN257693) and BA9
(DQ227395).