Enzyme-linked immunosorbent assay
Co-culture supernatant and plasma were collected, and IgG and TNF-𝛂
level were determined by ELISA using the Mouse IgG or TNF-𝛂 ELISA Kit
according to the manufacturer’s instructions (Multisciences).
Statistical
analysis
GraphPad Prism software (v.8.0, San Diego, CA) was used to analyze.
Student’s t-test and one-way analysis of variance were used for
comparisons between two groups or multiple groups, respectively. For
clinical assessment, two-way ANOVA was used to determine significant
differences in arthritis scores. P < 0.05 was
considered statistically significant. P ‐values are depicted as
follows: *P < 0.05; **P < 0.01;
***P < 0.001.