2.3 Neutralizing antibody assays
Serum samples from participants were initially 10-fold diluted and
subsequently 3-fold serially diluted. The diluted serum samples were
then combined with an equal volume of GX_P2V (100
TCID50, GenBank accession number MW532698) incubated at
37℃ in a 5% CO2 incubator for 2 hours. Following
incubation, 100 µL of the virus-serum mixture was transferred into
96-well plates and combined with an equal volume of BGM cells
(3×104 cells/well). The inoculated plates were
incubated continuously at 37℃ in a 5% CO2 incubator for
an additional 5 days, after which the cytopathic effect (CPE) of the
virus were observed using a light microscope. GX_P2V neutralization
titers were calculated using the Reed-Muench equation and expressed as
the sample dilution at which a 50% neutralization titer
(NT50) was achieved.