2.10. Drug screening analysis and biosensor sensitivity study
The drug toxicity of the LOC platform was determined by incubating it
with different concentrations of diclofenac (DF) (1, 10, 100, 200, 400,
800, and 1000 μM) (Sigma) for 48 h. After treatment, the cell viability
of the LOC was assessed using the CCK-8 assay, and measurements were
performed calorimetrically. To determine the biosensor sensitivity, CA
measurement of the drug-treated LOC was performed.