Isolation of CD8+ T cells with magnetic beads
kits
The CD8+ T cells from PBMCs were isolated by magnetic
streptavidin nanobeads of Biolegend MojoSortTMIsolation Kits. Incubating PBMCs with biotin antibody cocktail followed
by incubation with magnetic streptavidin nanobeads. Targeted cells were
depleted by magnetically labeled fraction using a magnetic separator,
while untouched CD3+CD8+ cells were
collected.