K562 cell lines transfected with HLA-E molecule
An immortalized chronic myelogenous leukemia cell line K562 with MHC molecules expression deficiency was obtained from ATCC (CCL-243). We stably transfected K562 cell lines with single allele HLA-E*0103 or HLA-E*0101 to generate mono-allelic cell lines with lentivirus transfected methods. Stably transfected cells showed strong expression (>95%) of HLA-E*0103 or HLA-E*0101 according to flow cytometry (FCM) analysis.