K562/HLA-E cells pre-incubation with peptides
Before incubation with peptides, K562/HLA-E*0103 cells were incubated at
26℃ for 24 hours to adapt to the condition for antigen presentation in
advance. K562 cells were then incubated with peptides constructed (ul/10
cells) at 26℃ for 19h for the next assay.
Ex Vivo IFN-γ
enzyme-linked immunospot assay (ELISPOT)
The human IFN-γ ELISPOT kit (DAKEWE Biotech Company, China) was used to
detect the capacity of IFN-γ production by CD8+ T
cells stimulated with specific antigenic peptides presented by
K562/HLA-E cells as previously. The spots representing peptide-specific
IFN-γ-producing CD8+ T cells were counted using an
automatic ELISPOT reader (Cellular Technology Limited, USA). Adjusted
spot-forming cells (SFCs) after subtracting negative values are
expressed as SFCs/106 cells.