T cell immune responses analysis
Mouse splenocytes cultured in RPMI 1640 (Invitrogen) containing 2 mM L-glutamine, 50 U/mL penicillin, and 10% fetal bovine serum (Invitrogen) were stimulated with 15 μg/mL rHBsAg or 10 μg/mL recombinant HBcAg (Catalog #hbv-232-a, ProSpec, China) for 72 h. Interferon gamma (IFN-γ) in culture medium was measured using ELISA kits (Catalog #D721025, Sango, China).