T cell immune responses analysis
Mouse splenocytes cultured in RPMI 1640 (Invitrogen) containing 2 mM
L-glutamine, 50 U/mL penicillin, and 10% fetal bovine serum
(Invitrogen) were stimulated with 15 μg/mL rHBsAg or 10 μg/mL
recombinant HBcAg (Catalog #hbv-232-a, ProSpec, China) for 72 h.
Interferon gamma (IFN-γ) in culture medium was measured using ELISA kits
(Catalog #D721025, Sango, China).