Conclusions
Wet-interface matrix deposition devices for MALDI mass spectrometry imaging are convenient and highly effective in use. Proper optimization of parameters such as the number of matrix layers and the nozzle height over the sample allows substantial improvement of the analysis parameters. Thus, the analysis of precious biological samples can be performed with the best possible quality. However, the care must be taken during the matrix selection to visualize the molecules of interest without losing their spatial distribution. Moreover, our analysis shows that the ion map obtained with the aid of a particular matrix might differ significantly with the use of another. So, care must be taken when comparing the results and drawing biological conclusions from such analyses.
Funding: This work was supported by the Polish National Science Center (NCN) grant number 2018/29/B/NZ4/02243 to ABK, statutory funds from the Maj Institute of Pharmacology at the Polish Academy of Sciences to PM, subsidy no 16.16.160.557 of the Polish Ministry of Science and Education to ABK, PS, PK and IK.
Institutional Review Board Statement: The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Local Ethics Committee (agreement number 137/2018).
Data Availability Statement: All the data present in the study are available upon request from the corresponding author.
Conflicts of Interest: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.