Vascular network labeling in transparent tissues provides more complete
information on blood vessels. To achieve a fast and efficient method for
vascular network labeling in transparent tissues, we compared various
vascular labeling methods under different tissue clearing protocols.
FITC-dextran labeling and CUBIC cleaning treatment were found to be the
best options for vascular network labeling in cleared mouse tissues.
Satisfactory labeling of vascular networks in various organs can be
achieved by selecting FITC-dextran with different molecular weights and
different administration methods.