Preparation and characterization of drug-loaded nanoparticles
Rifampicin-loaded nanoparticles were prepared through single emulsion evaporation method, as previously standardized (108), with slight modification in preparation of radio-labeled rifampicin nano-particles. DCM was evaporated in rota-evaporator for 15minutes and drug loading was assessed by using a well counter (Captus 4000e) present in the Nuclear Medicine Department in our institute.