Sample collection for pharmacokinetics of rifampicin and
bio-distribution of radio-labeled rifampicin
Participants remained at the clinical site from 10 h prior to the
administration of the study drug on day 1 till “the completion of all
post-dose sample collections on day 8. Blood was collected at 0
(pre-dose), 0.5-1, 2”, 6, 24, 48, 72, 96, 120, 144, and 168 h after
dose from an indwelling IV catheter. Time and date of dose
administration, exact volume of the sample were noted. The blood samples
were divided into two aliquots of volume 2 ml, one for radio-active
counts another for the analysis of rifampicin from whole blood.
“Participants were asked to void their bladder in the morning prior to
dosing, from which the pre-dose urine sample was obtained. After dose
administration, participants were asked to note the total volume of
urine whenever they voided the bladder. One ml of urine and 1 ml of
blood samples were stored for radioactivity measurement. The
time-activity curve was plotted using the OLINDA software.