Assessment of quality of radio-labeled rifampicin.
The HPLC method was used to investigate the labeling of rifampicin. Analysis was performed on the HPLC (Shimatzu, SPD 2A LC-20AD), using a reverse phase ultrabase-C18 column (250mm4.6mm, 5m particle size). The mobile phase ratio was 70:30 v/v of 0.05M disodium phosphate and ACN at 1 ml/min flow rate, 25°C oven temperature, and 335 nm wave length.