Drug estimation by LCMS-MS
Levels of rifampicin in the formulation and plasma samples were assessed using LCMS/MS (SCIEX triple Quad 35000). Mobile phase consists of ACN and 5mM ammonium formate with 0.1% formic acid for ionization in a ratio of 80:20, flow rate 1ml/minute, coloum C18(Nucleosil-100-3, 150/4.6 mm, 3µm) and injection volume 10µl.