Materials
PLGA (50:50) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Rifampicin was purchased from HiMedia, India, and PVA (MW: 30000-70000 Da, 88% hydrolyzed) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Acetonitrile (ACN; LCMS grade) and dichloromethane (DCM) were purchased from Merck Ltd.(Mumbai, India). Dialysis bags (cut-off of 12 kDa) were purchased from HiMedia, India.