Figure Legends
Figure 1.
Results of diagnostic and radiotherapy simulation imaging throughout the disease course. Axial computed tomography (CT) images are shown, corresponding to the timeline showing therapy. White arrows indicate left-neck lymph node metastasis, and red circles indicate abdominal para-aortal lymph node metastasis. (A) Representative figures before treatment with nivolumab. (B) Figures showing enlargement of the left-neck lymph node and abdominal para-aortal lymph node metastases. (C) Figure showing the CT simulation image for radiotherapy planning. The isodose paint represents total doses of >40 Gy (red), 20–36 Gy (green), and 12–20 Gy (blue). (D) Figures showing findings 3 months after radiotherapy. The irradiated left-neck lymph node metastasis regressed to a scar-like appearance. Furthermore, disease response outside of the radiation field was seen.
Figure 2.
(A) Enrichment of the T-cell receptor (TCR)/B-cell receptor (BCR) pool in clones. There are slight increases in the percentage of unique clones accounting for >0.01% of total TCRs/BCRs before and after radiotherapy, and large increases in that of unique clones accounting for >0.1% of total TCRs/BCRs. (B) Top 10 TCR/BCR clones after radiotherapy. In the TCR repertoire, the top 10 clones are mostly similar to that before radiotherapy, but there are some changes (the percentages of the 1st, 2nd, and 4th clones increased more than 0.5%). In the BCR repertoire, all the top 10 clones before radiotherapy are replaced after radiotherapy. Furthermore, 9 of the 10 clones are newly detected after radiotherapy.
□: before radiotherapy; ■: after radiotherapy; *: not detected