Result:
The degree of concordance of RBC, WBC and epithelial cells was good between both Automated analyzers (Sysmex UC-3500 -UF 5000 Urine Chemistry Analyzer and Super+ YD Diagnostic Urine analyzer ) and routine microscopy with p <0.001. The concordance for formed elements and cells between the Sysmex UC-3500 -UF 5000 Urine Chemistry Analyzer and the manual microscopy was better than the degree of concordance between the manual microscopic method and the Super+YD Diagnostic Urine analyzer. (Table 1, Table 2). The agreement of the Sysmex UC-3500 -UF 5000 with manual microscopy was almost good (κ > 0.6) for erythrocytes and leucocytes and moderate (κ = 0.41-0.60) for squamous epithelial cells. There was moderate agreement (κ = 0.41-0.60) for WBC, RBCs and squamous epithelial cells for URiSCAN Super+ YD Diagnostics analyzer and microscopy. The agreement of both the Analyzers was very good for sugar, ketone bodies and protein (0.81-1.00). There was no concordance observed between all the methods for crystals, cast, bacteria and fungi with p >0.05. Both the automated analyzers showed similar results to the manual microscopic examination for red cells, white blood cells and epithelial cells.(Fig 1,2,3,4) However, bacteria, fungi, dysmorphic cells, casts and crystals need to be analyzed by manual microscopy for a definite diagnosis. There is a need for further upgradation of the software programs for accurate results and better concordance with microscopy.