Conclusions
The addition of one (or two) intermediate temperature program steps to the method developed by Golay et al [7] for the analysis of FA in dairy products and infant formula extends the method to the analysis of PHO and RVO. While the addition of an isothermal plateau at 184°C (method 1) achieves the separation of trans -18:3 fromc 11-20:1, the second proposed modification including a negative temperature gradient also provided greater separation betweent 13/t 14-18:1 and c 9-18:1 (method 2).