Supplementary figure 2 The effect of TNF-α and IL-5 on ICOSL expression of purified eosinophils
(A). The purity of isolated eosinophils verified by Flow cytometry (left panel) and Wright-Giemsa staining (right panel, x 1000). (B). The expression level of CD40 and ICOSL on purified eosinophils at 0h accessed by flow cytometry. (C). The effect of TNF-α and IL-5 on ICOSL expression of eosinophils isolated from healthy donators. GM-CSF (50 ng/mL) stimulation used as basic condition (medium) for all groups. Freshly isolated eosinophils were cultured with medium alone, or rhTNF-α (50 ng/mL), or rhIL-5 (50 ng/mL) or rhTNF-α plus rhIL-5. ICOSL expression was then detected by Flow cytometry.