The drivers of tree growth are one critical question in forest ecology and conservation. However, the measurement of tree growth is a difficult task that requires novel methods to improve accuracy and broaden the understanding of the effect of climate on tree metabolism and carbon accumulation. In this context, isotopes variation along woody tissues is a strong tool that provides new information about tree metabolism, growth rate, and the effect of climatic variation on these processes at high temporal resolution. Here, we obtained woody samples of two tree species two individuals per species (n = 4) from the Biogeographic Choco; Region in Colombia, one of the most humid regions of the planet without dry periods (mean annual temperature 25.9C and rainfall over 7200 mm). We measured 18O and 13C on these samples across some rings in each one to obtain intra-annual variation. Using these data, we assessed if isotopes variation in wood is correlated with climatic variation, explicitly precipitation regimen indicators employing Pearson correlation and linear mixed effect models. We found that both isotopes are correlated negatively with ring width. We also found that 18O is high negative correlated with precipitation indicators, rather than 13C. Our results suggest that isotopes variation are surrogates of tree growth in humid and non-seasonal forests. Besides, the 18O accumulation, which is strongly related to rainfall during the less rainy month (February: 370 mm on average), could be a better indicator of the effect of precipitation on the woody tissue rate change. However, 13C is more related to tissue formation processes. In conclusion, we found evidence of intra-annual variation in isotopes and tree growth in one hyper-humid forest challenging the effect of the dry season of tree growth and potentially suggesting the water excess as an additional limiting factor controlling growth rhythms in tropical trees.