Effects of COX‑2 depletion on cAMP/PKA/CREB/BDNF signaling pathway.
Then we detect the expression of cAMP with Elisa, total and phosphorylated PKA/CREB and BDNF with western blot in the hippocampus of each group. As it illustrated in Figure 6A and B, COX-2KO mice showed lower levels of cAMP in comparison with WT mice, which were accompanied by a lower level of phosphorylated PKA in total PKA, phosphorylated CREB in total CREB, as well as BDNF. The result showed that the cAMP, p-PKA, p-CREB and BDNF can all be reduced in the hippocampus of COX-2KO mice.