Figure 4 Effects of auxotrophic genes from α-Ketoglutarate module on cell growth and FFA production. Control: prototrophic strain;his3 Δ: strain ScYan8 with single damage of gene HIS3 ;arg4 Δ: strain ScYan13 with single removal of gene ARG4 . Growth curve of his3 Δ and arg4 Δ were measured by adding exogenous histidine and arginine of 20, 60 and 100 mg/L, respectively (A and B). Fatty acid titers at fermentation endpoint (48 h) ofhis3 Δ and arg4 Δ were measured (C and D). All data was represented as the mean ± s.d. of three clone samples.