Genotyping
Three SNPs (UGT1A6 rs17863783, SLC28A3 rs7853758 and RARG rs2229774)
that previously showed strong evidence of association with DIC were
selected 9,15. Genomic DNA was extracted from whole
blood using the Applied Biosytems MagMAX DNA Multi-Sample Ultra 2.0 Kit
using an automated system (King FisherFlex Magnetic Particle Processor
System). Genotyping was done using the GenoPharm® pharmacogenomics open
array panel which contains 120 assays for over 48 genes. GenoPharm® uses
TaqMan Assay chemistry using the Applied Biosystems QuantStudio 12K Flex
Real-Time PCR System (Thermo Fisher Scientific, Waltham, Massachusetts).