Genotyping
Three SNPs (UGT1A6 rs17863783, SLC28A3 rs7853758 and RARG rs2229774) that previously showed strong evidence of association with DIC were selected 9,15. Genomic DNA was extracted from whole blood using the Applied Biosytems MagMAX DNA Multi-Sample Ultra 2.0 Kit using an automated system (King FisherFlex Magnetic Particle Processor System). Genotyping was done using the GenoPharm® pharmacogenomics open array panel which contains 120 assays for over 48 genes. GenoPharm® uses TaqMan Assay chemistry using the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, Massachusetts).