Sample preparation and sequencing
DNA was extracted using a modified phenol-chloroform extraction method
(Davies et al., 2013) and genotyped with the original version of the
2b-RAD protocol as described by Wang et al . (Wang, Meyer, Mckay,
& Matz, 2012). Six randomly chosen samples were genotyped four times to
provide a reference of what genetically identical samples should look
like in subsequent analyses. The libraries were individually barcoded,
pooled, and sequenced on six lanes of the Illumina HiSeq 4000 at UT
Austin. Adapter-trimmed reads have been deposited to NCBI-SRA,
bioproject PRJNA565239.