Molecular identification and phylogenetic tree construction
A total of 22 sequences, including 11 sequences of cox 1 and 11 sequences of ITS2, were obtained from 10 gorals and 1 serow. All samples were confirmed to be S. scabiei through BLAST searching (NCBI). All the sequences of cox 1 and ITS2 were submitted to GenBank (GenBank accession numbers: cox 1: ON428480-ON428489 and ON514171; ITS2: ON426154-ON426163 and ON514602).
The alignment results showed that 11 cox 1 sequences and 11 ITS2 sequences of S. scabiei collected from gorals and serows in different areas had 98.6% to 100% and 99.1% to 100% identity, respectively (Fig. 5A and B). The phylogenetic tree of cox 1showed that the 11 sequences were within the clade of S. scabieithat originated from rabbits in China (Fig. 5C).