Key points
Liquid-liquid phase separation emerges as a universal tool for the
intracellular biomolecule organization, particularly via the formation
of membraneless organelles.
The liquid-like membraneless organelles afford unique properties and
have aroused wide interests among cell biologists.
Metastability is a general feature of liquid-like membraneless
organelles, which has manifested in multiple aspects including energy
state, molecular interactions, molecular structures, biological
assemblages and its association with diseases.
Understanding of metastability of biological liquid phase separation can
not only help to elucidate the formation and properties of membraneless
organelles, but also pave the way to the insight into pathogenesis.