3.1 nrITS and cpDNA dataset acquisition and assessment
Twelve ITS ribotypes were identified based on 16 variable nucleotide
sites. The ITS1/2 length was 701 bp, nucleotide diversity (π) was
0.00390, the average number of nucleotide difference (K) was 2.681, and
haplotype diversity (Hd) was 0.810 (Table 2). The geographical
distribution of ribotypes showed that only a few ribotypes were shared
across the species distribution range, whereas most of them were private
to a single population (Table 1, Figure 1).
The two cpDNA sequences used contained 50 polymorphic sites, and yielded
19 chlorotypes. Nucleotide diversity (π) was 0.00860, average number of
nucleotide difference (K) was 11.634, haplotype diversity (Hd) was 0.919
(Table 2). All chlorotypes were exclusive to a single or a group of
neighbouring populations (Table 1, Figure 1). The total length of the
combined chloroplast alignments was 1404 bp (730 bp and 674 bp in size
for trn L-trn F and ycf 1b, respectively), a
congruency test implied a high level of homogeneity of the two
chloroplast sequences (P > 0.5) and a striking
non-homogeneity between nrDNA and cpDNA (P < 0.5).
Ribotype and chlorotype diversities (Hd) of the 10 sampled populations
ranged from 0 to 0.586 and 0 to 0.711 respectively (Table 1).