Statistical analysis
All data were analysed using IBM SPSS Statistics for Windows, Version
22.0 (IBM Corp., Armonk, NY, USA) and GraphPad Prism version 5.0 for
Windows (GraphPad Software, San Diego, CA, USA) software. Analysed data
were expressed as numbers and percentages or mean ± standard deviation
values, where appropriate. Frequency analysis was used for statistical
evaluation and categorical variables were compared using chi-square
test. For continuous variables, normality of distribution was evaluated
by D’Agostino-Pearson and Shapiro-Wilk analyses. Normally distributed
data were compared using one-way analysis of variance (ANOVA) test with
Tukey’s post-hoc test, whereas Kruskal-Wallis test was used with Dunn’s
post-hoc test if normal distribution is not applicable. Statistical
significance was inferred by an overall 5% of type-I error level.