Statistical analysis
All data were analysed using IBM SPSS Statistics for Windows, Version 22.0 (IBM Corp., Armonk, NY, USA) and GraphPad Prism version 5.0 for Windows (GraphPad Software, San Diego, CA, USA) software. Analysed data were expressed as numbers and percentages or mean ± standard deviation values, where appropriate. Frequency analysis was used for statistical evaluation and categorical variables were compared using chi-square test. For continuous variables, normality of distribution was evaluated by D’Agostino-Pearson and Shapiro-Wilk analyses. Normally distributed data were compared using one-way analysis of variance (ANOVA) test with Tukey’s post-hoc test, whereas Kruskal-Wallis test was used with Dunn’s post-hoc test if normal distribution is not applicable. Statistical significance was inferred by an overall 5% of type-I error level.