Comparative analysis of mitochondrial genomes
The mitochondrial genomes of FCDK (GenBank accession No. OL672753) and FCSH (GenBank accession No. OL672754) were de novo assembled from Illumina raw reads (approximately 6 GB raw data) using NOVOPlasty v2.7.2 (Dierckxsens et al., 2017). The assembled mitochondrial genomes were annotated via the MITOS2 web portal (Donath et al. 2019) and manually checked. The circular representation of the mitochondrial genome was plotted using the CGview Server (Grant & Stothard, 2008). The alignment of the two whole mitochondrial genomes was built using the Mauve plugin for Geneious Prime (Kearse et al. 2012), and detailed sequence identities were calculated with custom Python scripts.