Inter- and intra-genomic synteny
To reveal chromosomal evolution between FCDK and FCSH, syntenic blocks were detected using MCScanX (Wang et al., 2012) with an e-value of 1e-10 and a minimum syntenic size of 5 (‘-b 2 -s 5 -e 1e-10’). Protein sequence alignments were generated by MMseqs2 BLASTP-like searching in the sensitive mode (-s 7.5) with an e-value of 1e-5. Large chromosomal regions unique to FCDK (no syntenic blocks were defined in FCSH) were surveyed. GO and KEGG pathway enrichment analyses were performed for PCGs located in these regions using clusterProfiler v3.14.3 (Yu et al., 2012). The significance level was controlled to the default, with a p-value of the hypergeometric distribution of 0.01 and a q-value for the multiple comparison FDR of 0.05.