Inter- and intra-genomic synteny
To reveal chromosomal evolution between FCDK and FCSH, syntenic blocks
were detected using MCScanX (Wang et al., 2012) with an e-value of 1e-10
and a minimum syntenic size of 5 (‘-b 2 -s 5 -e 1e-10’). Protein
sequence alignments were generated by MMseqs2 BLASTP-like searching in
the sensitive mode (-s 7.5) with an e-value of 1e-5. Large chromosomal
regions unique to FCDK (no syntenic blocks were defined in FCSH) were
surveyed. GO and KEGG pathway enrichment analyses were performed for
PCGs located in these regions using clusterProfiler v3.14.3 (Yu et al.,
2012). The significance level was controlled to the default, with a
p-value of the hypergeometric distribution of 0.01 and a q-value for the
multiple comparison FDR of 0.05.