Comparative analysis of mitochondrial genomes
The mitochondrial genomes of FCDK (GenBank accession No. OL672753) and
FCSH (GenBank accession No. OL672754) were de novo assembled from
Illumina raw reads (approximately 6 GB raw data) using NOVOPlasty v2.7.2
(Dierckxsens et al., 2017). The assembled mitochondrial genomes were
annotated via the MITOS2 web portal (Donath et al. 2019) and manually
checked. The circular representation of the mitochondrial genome was
plotted using the CGview Server (Grant & Stothard, 2008). The alignment
of the two whole mitochondrial genomes was built using the Mauve plugin
for Geneious Prime (Kearse et al. 2012), and detailed sequence
identities were calculated with custom Python scripts.