Genome sequencing
Total genomic DNA of FCDK and FCSH was extracted from mixed eggs or
juveniles, respectively, using a DNeasy Boold & Tissue kit (Qiagen)
following the manufacturer’s protocol. The Illumina sequencing of FCSH
was performed by Geneworks (Suzhou, China) using the Illumina HiSeq X
platform. The Pacbio sequencing of FCSH and both the Illumina and PacBio
sequencing of FCDK were performed by Macrogen (Korea) using the Illumina
HiSeq X and PacBio Sequence RSII (P6C4 chemicals) platforms,
respectively. The Hi-C experiments were performed by Frasergen (Wuhan,
China) following the standard procedure using the Illumina HiSeq X
platform. The detailed statistics of all sequencing data are shown in
Table 1.