Sample collection and rearing
The parthenogenetic Danish strain FCDK was originally obtained in 2007
from Paul Henning Krogh’s lab at Aarhus University, Denmark, and the
sexual Shanghai strain of FCSH was collected in 2008 from Shanghai
Botanic Garden, China. Both strains have been cultured in our laboratory
for over 10 years. They are reared in Petri dishes containing a
solidified mixture of plaster of Paris and activated charcoal (9:1
wt/wt, dissolved in distilled water), fed granulated dried baker’s yeast
and distilled water, and kept in an artificial climate chamber in total
darkness (20 ± 1°C, 80% relative humidity).