The entropy estimated in this manner is considered as an upper limit due to the inherent assumption that the NH bond fluctuations for each residue are independent of others.
Acknowledgments
The NMR Research Centre supported by the Department of Science and Technology (DST), India, and the SPR facility at IISc are gratefully acknowledged. HSA acknowledges research grant support from DAE-BRNS. RSN acknowledges fellowship support by the National Health and Medical Research Council of Australia. SAR was supported by the NIH grant CA138313. GJ acknowledges support from IISc and CSIR. We acknowledge the assistance provided by Ms. Kerrie McNeil (University of Adelaide, Australia) in the preparation of labeled IGF-1, and thank Dr. Cameron Mackereth (IECB, Bordeaux, France) for his helpful suggestions to improve the quality of the manuscript.
References