Figure 1. Process overview
The whole process for rSaCas9 production and assessment comprises four
steps. The first step involves the cloning of constructs for the
production of rSaCas9 and sgRNAs, as well as the DNA target sequence.
The actual formation of rSaCas9, different sgRNAs, and the preparation
of plasmid DNA containing the target sequence are depicted in the second
column. Step three consists of the formation of the RNP complex and the
enzyme activity test on target DNA. The final step is the analysis of
the activity assay. Created with BioRender.com.