Figure 1. Process overview
The whole process for rSaCas9 production and assessment comprises four steps. The first step involves the cloning of constructs for the production of rSaCas9 and sgRNAs, as well as the DNA target sequence. The actual formation of rSaCas9, different sgRNAs, and the preparation of plasmid DNA containing the target sequence are depicted in the second column. Step three consists of the formation of the RNP complex and the enzyme activity test on target DNA. The final step is the analysis of the activity assay. Created with BioRender.com.