Figure 2. Production of rSaCas9 in BYL
Coupled in vitro transcription/translation was performed in cell-free BYL to produce rSaCas9. The transcription and translation of eYFP served as a positive control. A reaction without DNA template (NTC: no template control) was used as a negative control. After reaction times of 24 h and 48 h, 0.5 µl aliquots were separated by SDS–PAGE. Protein bands were stained by Coomassie Brilliant Blue. The filled arrowhead points to rSaCas9, and the open arrowhead indicates eYFP.