Mode 2: RBD4 forms the major component of the interaction
interface
This binding mode 2 features anticipated residues on RNP1 motif (K304,
F306, F308), the positively charged residues on β-2 strand (R291, R293)
of RBD 4, and on the linker loop between β-2 and β-3 (R298). This
binding mode is predicted in the mir-15a and mir-103a docking scenarios
(Fig 4 ) and suggests the involvement of multiple beta strands
from a single RBD, RBD4. The binding mode 2 bears resemblance to the
mir-18 binding mode of hnRNP A1 [9] (described in the next section),
both in terms of the composition and location of the residues involved
in the interaction and in the overall arrangement of the beta strands.
In both cases, the interactions are driven by residues on 3 beta strands
of the same RBD.