Figure Legend
Figure 1. Functional association between 2q variants and CTLA-4 expression. (A) Meta-analysis of whole blood eQTL association between CTLA-4 mRNA expression and rs3087243 genotypes, using 31,300 samples constituting 36 different cohorts from the eQTLgen consortium. (B) Schematic illustration of theCTLA-4 gene region with the relative location of SNPs. The cloned region and haplotypes used for the in vitro luciferase assay were also indicated. (C) Relative luciferase units (RLUs) of theCTLA-4 promoter construct were compared across haplotypes. Promoter-less luciferase plasmid pGL4.10 (empty vector, EV) was used as a background control. Lipo: Lipofectamine transfection reagent.(D) Protein expression of CTLA-4 in naïve-type CD45RA+ T regulatory (Treg) cells was compared across rs11571316 genotypes. An independent t-test was conducted to determine statistical significance in (C) and (D) that was defined as p -value < 0.05.