Figure Legend
Figure 1. Functional association between 2q variants
and CTLA-4 expression. (A) Meta-analysis of whole blood
eQTL association between CTLA-4 mRNA expression and rs3087243
genotypes, using 31,300 samples constituting 36 different cohorts from
the eQTLgen consortium. (B) Schematic illustration of theCTLA-4 gene region with the relative location of SNPs. The cloned
region and haplotypes used for the in vitro luciferase assay were
also indicated. (C) Relative luciferase units (RLUs) of theCTLA-4 promoter construct were compared across haplotypes.
Promoter-less luciferase plasmid pGL4.10 (empty vector, EV) was used as
a background control. Lipo: Lipofectamine transfection reagent.(D) Protein expression of CTLA-4 in naïve-type CD45RA+ T
regulatory (Treg) cells was compared across rs11571316 genotypes. An
independent t-test was conducted to determine statistical significance
in (C) and (D) that was defined as p -value
< 0.05.