Predictive Phylogeography
Before assessing whether these species harbor cryptic diversity, we made phylogeographic predictions of cryptic and non-cryptic for both species following the procedure introduced by EspĂ­ndola et al (2016) using Random Forest with bioclimatic variables associated with sample localities and taxonomic ranks. Following Sullivan et al. (2019), we also included trait values along with the bioclimatic and taxonomic variables. The error rates we recovered were congruent with those found by Sullivan et al. (2019) and thus these classifiers were used to make predictions about Thuja plicata and Tsuga heterophylla . Each classifier predicted neither species to harbor cryptic diversity (Table 2), with the only variation in the prediction being the less accurate classifier (bioclimatic data only).