Platelet RNA quality analysis and sequencing
Total RNA quality and quantity was assessed using the Agilent
Bioanalyzer 2100 and the Qubit fluorometer, respectively. Samples with
sufficient quantity and quality RNA went on to poly(A) mRNA capture and
construction of stranded mRNA-seq libraries from total RNA was achieved
using the commercially available KAPA Stranded mRNA-Seq Kit (catalog
#KK8421) using the manufacturer’s protocol and as described in the
Supplemental Methods.